Oxford Colloid Group

Microfluidic devices are a powerful tool in biological sciences. Different microfluidic techniques have contributed to the improvement of existing biological assays and to the development of novel quantification methods. Droplet-based microfluidics is a technique that is becoming increasingly important for various applications such as single cell analysis, complex biochemical assays, drug screening and directed evolution experiments. 

Micro-droplets can be considered as very small reactors with volumes of the order of a picolitre, which can be generated and manipulated in different ways. Various components can easily be encapsulated upon droplet formation and additional reagents can be added post-formation. In collaboration with the group of Prof. John Frater in the Nuffield Department of Medicine in Oxford we are applying droplet-based microfluidics techniques study of HIV-1 infection, and are currently setting up a system to sort rare HIV-1 infected cells encapsulated in droplets. These cells are particularly interesting since they persist during antiretroviral therapy and are the major obstacle to the eradication of the virus.  Single-cell techniques are also being used to benchmark immune repertoires and profile the effect of vaccination and convalescence in the context of COVID-19.

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Last updated 29/01/2022.